Journal: Frontiers in Oncology

Article Title: PSCA-directed nanosized bio-immune conjugates (NANO:BICs) enable selective uptake of TLR9 agonists in bladder cancer cells

doi: 10.3389/fonc.2026.1739618

Figure Lengend Snippet: Fluorescence labeled cells of the cell line SW780 and channels of separately stained components (top) and merged image (bottom). For visualization of cell nuclei DAPI-staining was used. The DAPI fluorescent stain was detected in the blue channel at 457 nm. The fluorescent label FITC was used in conjugation with the ODN as a labeled NANO:BICs component and detected in the green channel at 488 nm. WGA was used to label cell membranes and detected in the red channel at 647 nm.

Article Snippet: The BCa cell line SW780 (ATCC), derived from a grade 1 BCa, with endogenous expression of PSCA was cultured in DMEM complemented with 4.5 g/l glucose supplemented with 10% v/v heat-inactivated FBS ( ).

Techniques: Fluorescence, Labeling, Staining, Conjugation Assay

Journal: Frontiers in Oncology

Article Title: PSCA-directed nanosized bio-immune conjugates (NANO:BICs) enable selective uptake of TLR9 agonists in bladder cancer cells

doi: 10.3389/fonc.2026.1739618

Figure Lengend Snippet: Uptake of the NANO:BICs and ODN in different treatments of the SW780 cell line. The cells were seeded in an 8-well culture slide at a cell number of 8750 cells per well. After 72 h incubation in an incubator at 37 °C, the cells were incubated with fluorescently labeled NANO:BICs or ODN. 24 h after the start of stimulation, the cells were fixed. Cell components were stained after fixation and microscoped together with the stained NANO:BICs. The graph measures the integrated density (IntDen) per cell as a quantitative metric used to assess fluorescence intensity. This indicated the level of the fluorescent marker FITC in each cell as the intensity measured over the area of the cells in an image was divided by the number of cells present in this image for standardization. N = 20; MW ± SD; For all analyses, a two-tailed test was performed, and a p-value of less than 0.05 was considered statistically significant. In the figure, brackets are used to denote the specific groups being compared. The corresponding p-value thresholds are indicated above the brackets using the following convention: p ≤ 0.001 (***); and p ≤ 0.0001 (****).

Article Snippet: The BCa cell line SW780 (ATCC), derived from a grade 1 BCa, with endogenous expression of PSCA was cultured in DMEM complemented with 4.5 g/l glucose supplemented with 10% v/v heat-inactivated FBS ( ).

Techniques: Incubation, Labeling, Staining, Fluorescence, Marker, Two Tailed Test